Abstract:Objective To establish and identify the VSC4.1 cell line that stable-expressing hSOD1G93A. Methods The pCI-neo-Vector, pCI-neo/WT-SOD1 and pCI-neo/G93A-SOD1 was transfected into VSC4.1 cell by activated-dendrimer structure，the stably transfected cells were screened by G418. The expression of hSOD1 in VSC4.1 cell was detected by Western Blotting. The growth of the cell line we established was assessed by MTT assay. Results Both VSC4.1-hSOD1WT and VSC4.1-hSOD1G93A expressed hSOD1 according to the result detected by Western Blotting, and the VSC4.1-mock did not express that at all. The viability of VSC4.1-hSOD1G93A was lower than VSC4.1-mock (P =0.031, P =0.000） and VSC4.1-hSOD1WT（P =0.001, P =0.000） at 48h, 72h;there was no significant difference at other time points (P >0.05). Conclusion The VSC4.1 cell line stable-expressing hSOD1WT and hSOD1G93A was established，and it could be the foundation of the exploration for therapy and pathogenesis of amyotrophic lateral sclerosis (ALS).