Objective To provide a reference for studies of monitoring lung function in mice, this study was aimed to test the indexes of a non?invasive measurement of lung function in mice. Methods Lung function indexes of 460 C57BL/6 mice were detected by whole?body plethysmography. The results were analyzed, and the range of reference values was determined by the percentile method. Results Normal ranges for the following measures were found: inspiration time was 64. 7 (55. 30 -82. 60) ms, expiration time was 83. 4 (71. 70 -109. 20) ms, Rpef was 0. 21 (0. 16 -0. 28) ms, end?inspiratory pause was 2. 19 (1. 96 -3. 76) ms, end?expiratory pause was 1. 67 (0. 12 -9. 15) ms, tidal volume was 0. 44 (0. 25 -0. 58) mL, enhanced pause was 1. 29 (0. 91 -2. 00) ms, pause was 1. 18 (1. 00 -1. 64) ms, expiratory flow?50 was 0. 64 (0. 30 -0. 98) mL/ s, relaxation time was 39. 0 (32. 40 -51. 50) ms, peak inspiratory flow was 9. 74 (5. 33 -12. 83) mL/ s, peak expiratory flow was 9. 86 (5. 12 -13. 47) mL/ s, inspiratory frequency was 412 (331 - 474) BPM, and minute volume was 174. 4 (86. 69 -235. 04) mL. Conclusions The normal reference ranges from non?invasive lung monitoring in C57BL/6 mice can be used as a reference for basic research on respiratory diseases.