Objective To establish a porcine model of liver failure after different percent hepatectomy. Methods The porcine models of liver failure 75%, 85%, 95% hepatectomy were developed and the living conditions and survival time were recorded. The blood samples of pre-surgery, post-hepatectomy d1, d3, d5 and post-hepatectomy 1 week, 2 weeks, and 3 weeks were collected for hepatic function analysis. Histological examination of liver tissues was performed using HE staining. Liver injury histology was interpreted and scored in the terminal samples. Results The average survival time of pigs with post-hepatectomy liver failure after 75%, 85%, 95% hepatectomy was 19.0±5.6 days, 17.3±5.5 days, 1.3±1.5 days, respectively. Their pathological scores were 5.67±0.52, 8.17±0.82 and 8.50±0.71, respectively. With the increase of percent hepatic resection, the incidence of hepatic failure was increasing. ALT, AST, ALP, LDH and TBA were dramatically increased in the pigs after 85% hepatectomy. Conclusions The pig model of acute liver failure by 85% hepatectomy is successfully established, which can cause typical acute liver failure in Bama miniature pigs.