Zebrafish, a new type of model animal, has been widely used in many fields of biological research because of its low cost, ability of external fertilization, high fecundity, allowance of embryo transplant, and ectogenesis. Recently, zebrafish and its embryos have been widely used in ecotoxicological studies and environmental monitoring. Furthermore, with the maturation of zebrafish transgenic techniques, a new era has come for environmental pollution monitoring.