Objective To prepare liposomes of glycyrrhizic acid, and evaluate its liver targeting property in mice. Methods The liposomes were prepared with conventional rotary-evaporated film-ultrasonication method. The liposomes were injected into the mouse tail vein, and the concentration of glycyrrhizic acid was detected by RP-HPLC. The glycyrrhizic acid injection was taken as control. The targeted indicators, including the relative tissue exposure (r_e), targeting efficiency (t_e), and index of peak concentration ratio (C_e), were used to evaluate the liver targeting property. Results The r_e was 1.4, T_e of the liposomes was 0.092, and t_e of the injection was 0.059. The C_e of the liver was 1.59, and the C_e of the blood was 0.99.Conclusion Compared with glycyrrhizic acid injection, the glycyrrhizic acid liposomes show good liver-targeting property.