Objective To explore the establishment methods of animal models of intestinal multidrug-resistant bacterial (Pseudomonas aeruginosa, PA) infection, and to provide a good model for experimental research and treatment for infection caused by drug-resistant bacteria.Methods Male BALB/c mice were divided into four groups: Normal control group, MDR-PA group, MDR-PA + antibiotics group, and MDR-PA + fasting group. They were given saline, PA suspension, PA suspension after free drinking with ceftriaxone water and PA suspension after fasting, respectively, by gavage. Colon tissues were collected for pathological examination and inflammation scoring. The expressions of TNF-α and INF-γ in the colon tissue was assessed at the end of the experiment. Results Pathology showed different degrees of inflammatory changes in the colon tissues. Compared with the normal control group, the expressions of TNF-αand INF-γ had significant differences with that of experimental groups. Conclusions All the three methods used in this experiment can be successfully used to establish mouse models with intestinal infection caused by multidrug-resistant Pseudomonas aeruginosa, and may be chosen for experimental studies according to different research purposes.