Objective To establish a novel rat model of irritable bowel syndrome(IBS) by exposing to chronic unpredictable stressors.Methods Thirty male SD rats were used in this study.The rats were exposed to chronic unpredictable stress with additional individual housing to induce IBS symptom.The stress factors include:drink prohibition,fasting for 24 hours,day and night reversal,cold,heat,pain,arrest and swing.Visceral sensation was assessed by pressure threshold of abdominal withdrawal reflex(AWR),gastrointestinal ...