Objective To investigate whether HCMV infection can cause ocular tissue lesions in rats and mice.Methods Twenty rats and ten mice were inoculated with HCMV AD 169 strain by vein and development of ocular disease was observed.HCMV DNA of tissues from inoculated animals were examined by in situ hybridization.Results Some animals expressed much secretion,turbidity in eyes even losing sight at different time post\|inoculation.HCMV DNA sections were identified in the corneal endothelium cell,rod cell and cone cell of the rats and mice inoculated. Conclusion HCMV AD 169 strain can infect rats and mice and cause ocular disease.\;