Abstract:As an endogenous chemical substance, neurotransmitter plays a vital role in maintaining normal life activities of people. Abnormal levels of neurotransmitters can lead to physical, mental and some neurodegenerative diseases. However, the ultra-low concentration, complex chemical properties and release modes of neurotransmitters make their accurate detection in vivo a great challenge.In order to accurately monitor neurotransmitters in the brain and accurately understand the release kinetics of neurotransmitters, several commonly used methods for detecting neurotransmitters in vivo in the past five years and their research progress were reviewed.The basic principle and applicability of microdialysis, electrochemical sensor and fluorescence sensor are introduced in detail.