Objective A stable model of acute obstructive suppurative cholangitis in rats was established to detect its pathophysiological indexes and provide a stable and reliable standardized animal model for the study of acute cholangitis and cholestasis.Methods SPF grade male SD rats were selected and the model was constructed by injection of toxoid into the lower bile duct and ligation of the common bile duct, and the changes in body weight, mortality, major indexes of liver function and histopathological changes in the liver were evaluated before and after modeling.Results After modeling, the body weight of rats in the model group decreased significantly; the number of rats died was less; the main indexes of liver function and liver pathology showed obvious changes of cholestasis and liver function damage.Conclusion In this study, a rat acute obstructive suppurative cholangitis model was successfully established, which has the advantages of easy operation, low damage, low mortality and high successful modeling rate, and can provide a standardized experimental animal model for the study of the mechanism of many common diseases and drug development.