Objective To investigate the current status of mouse euthanasia methods in China, and to provide the reference and basis for the administrative authorities of laboratory animal management to master the implementation of "euthanasia" and formulate related policies. Methods Research papers containing the terms of "mouse" and "execution" in Chinese characters during the period from 2015 to 2016 were searched in Wanfang database, and statistical analysis was performed with the articles meeting the searching criteria. Results A total of 890 research articles met the searching criteria, of which 351 articles clearly described the killing method, accounting for only 39.44%. The mouse-killing methods included cervical dislocation, decapitation, exsanguination and sampling after anesthesia, excessive anesthesia, abdominal aorta bleeding and carbon dioxide asphyxiation, among them cervical dislocation accounted for the highest rate, 75.78%. Conclusions The current implementation of mouse euthanasia methods in our country has been far from optimistic. The mouse euthanasis methods have often been ignored in scientific articles and the description of the methods is not standardized. In order to promote the effective implementation of the regulations related to mouse euthanasia, it is needed to promote the study of related techniques and to strengthen personnel training.