Objective To explore the impact of music of different frequencies on the spatial learning and memory ability of mice. Methods A total of 80 healthy 6~8 weeks old C57 mice were randomly divided into 10 groups, half male and half female in each group. The mice in the control group were not stimulated by music. The mice of the 3 groups of high-frequency music, 3 groups of medium-frequency music and 3 groups of low-frequency music were stimulated by music in corresponding range of frequency, respectively, for 5 h each day. After 6 d, the ability of spatial learning and memory of the mice was tested in the Morris water maze, with the same stimulation of music during the test. The indicators of the test included the avoiding latency, the frequency of passing the platform and the time spent to pass the quadrant of the mice in each group. Results Compared with the control group, the avoiding latency of the mice in the groups with stimulation by music was significantly shorter (P< 0.05). The frequency of passing the platform and the time spent to pass the quadrant were increased, yet the difference was not significant (P> 0.05). Moreover, there was no significant difference between the indicators of the 9 groups receiving music stimulation (P> 0.05). Conclusions The 9 pieces of music we have selected can improve the ability of spatial learning and memory in adult mice, however, the effects of the music of different frequencies did not show significant difference. It is hypothesized that the impact of music stimulation on mice may be related to the criteria for music classification, the duration of cycle of music stimulation and the selection of time points of stimulation.