静脉注射大肠杆菌制备高动力型感染性休克犬模型
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大连市卫计委2013年课题项目(课题名称:《亚低温治疗感染性休克所致ARDS的实验研究》)。


Establishment of a dog model of hyperdynamic septic shock by intravenous injection of Escherichia coli
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    目的 通过静脉注射大肠杆菌方法制备高动力型感染性休克犬模型。方法 杂种犬22只,随机分为对照组(n=11)和实验组(n=11)。麻醉犬后,经右股静脉置入双腔中心静脉导管,对照组使用微量泵向犬股静脉内泵入生理盐水,实验组以同样方法泵入大肠杆菌,经右股动脉置入PICCO导管,在0~48 h各时点应用PICCO监测全身血流动力学情况。结果 实验组的心率(HR)、心排量(CO)、每搏量(SV)在12 h均显著升高(P< 0.05),收缩压(SBP)、外周血管阻力(SVR)在12 h显著降低(P < 0.05),而其他血流动力学指标如中心静脉压(CVP)、肺动脉楔压(PAWP)、氧输送(DO2)、氧消耗(VO2)、氧摄取率(O2ER)差异均无显著性(P> 0.05)。实验组从12 h起即可观察到明显的尿量减少;动脉收缩期峰值血流速度(PSV)和阻力指数(RI)与对照组相比差异均有显著性(P< 0.05)。结论 运用微量泵向犬股静脉泵入活大肠杆菌可以成功制备高动力型感染性休克犬模型,可为研究感染性休克提供实验基础。

    Abstract:

    Objective To establish a dog model of hyperdynamic septic shock by intravenous injection of Escherichia coli. Methods Twenty-two hybrid dogs were randomly divided into control group (n=11) and experimental group (n=11). A double-cavity central venous catheter was inserted into the right femoral vein of the dogs after anesthesia. Physiological saline was continuously pumped into the femoral vein of the dogs in the control group by the micro-pump, while the dogs of the septic shock group were pumped with E.coli in the same way. The catheter of PICCO (pulse indicator continuous cardiac output) was inserted into the right femoral artery and the systemic hemodynamics of the dogs was monitored at all of the time points from 0 to 48 h. Results Compared with the control group, the heart rate (HR), cardiac output (CO) and stroke volume (SV) of the experimental group were significantly increased at 12 h (P < 0.05), and the systolic blood pressure (SBP) and systemic vascular resistance (SVR) were significantly decreased at 12 h (P < 0.05). However, other hemodynamic parameters such as the central venous pressure (CVP), the pulmonary artery wedge pressure (PAWP), the oxygen delivery rate (DO2), the oxygen consumption rate (VO2), and the oxygen extraction rate (O2ER) were not significantly different (P > 0.05). Moreover, significant decrease in urine output was observed in the septic shock group since 12 hours, and the peak systolic velocity (PSV) and the resistance index (RI) of the systolic artery were significantly different from those in the control group (P < 0.05). Conclusions The dog model of hyperdynammic septic shock can be successfully established by intravenous injection of Escherichia coli into the femoral vein by a micro-pump. This model provides an experimental basis for further study of septic shock.

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崔嵩,韩叶叶,刘思伯.静脉注射大肠杆菌制备高动力型感染性休克犬模型[J].中国比较医学杂志,2017,27(9):48~52.

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  • 收稿日期:2017-01-20
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  • 在线发布日期: 2017-09-23
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