Objective To evaluate the sensitizations of Hydroxyethyl Starch 40 Sodium Chloride Injection. Methods Active systemic anaphylaxis(ASA) test, internal sensitive index's determination and screening and Passive Cutaneous Anaphylaxis(PCA) test are carried out. Observe the allergy safety of the samples. Results We use guinea pigs to carry on ASA test and PCA test with Hydroxyethyl Starch 40 Sodium Chloride Injection from 45 batches of 3 companies and no changes have occurred. Measuring and comparing the sensitive index of HIS,IgG, IgM and IgE in plasma, IgM and IgE are not obvious variation; HIS and IgG positive group and negative group and sample groups are different.Conclusions It is suggestion that Hydroxyethyl Starch 40 Sodium Chloride Injection safety evaluation should include allergic reactions, HIS and IgG sensitive index monitoring in clinical application of hydroxyethyl starch.