补肾养血方对卵巢早衰大鼠中抗苗勒氏管激素的影响
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

河南省中医药管理局科研课题(2014ZY02013)。


Effect of bushenyangxue prescription on the levels of anti-mullerian hormone in rat with premature ovarian failure
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的 建立雷公藤多苷致卵巢早衰大鼠的模型,研究补肾养血方对卵巢早衰大鼠中抗苗勒管素(anti-mullerian hormone,AMH)的影响。方法 使用雷公藤多苷建立卵巢早衰大鼠模型,造模成功后,分别采用高,中,低剂量补肾养血方对大鼠灌胃,每日一次,共30 d。给药完毕后于显微镜下观察各组大鼠卵巢组织形态。酶联免疫吸附法、免疫组化染色法和定量反转录聚合酶连锁反应(qRT-PCR)分别检测大鼠血清中AMH含量以及卵巢内AMH的蛋白和mRNA表达水平。结果 组织形态学显示高中低剂量组的卵泡和黄体的生长均比模型组好;酶联免疫吸附法、免疫组化和qRT-PCR结果显示补肾养血方呈剂量依赖性逆转雷公藤多苷导致大鼠血清中AMH浓度以及卵巢中AMH表达水平的降低。结论 补肾养血方可逆转雷公藤多苷引起卵巢中AMH水平降低,提示补肾养血方具有防止卵巢早衰的功效。

    Abstract:

    Objective Establish premature ovarian failure (POF) model in female Sprague Dawley by tripterygium wilfordii, and investigate the effect of bushenyangxue prescription on the levels of anti-mullerian hormone (AMH). Methods After POF model was established, rats were gave by gavage of different dosage of Bushenyangxue prescription for 30 days. The changes of histomorphology on rat ovarian tissue were observed by hematoxylin-eosin staining. Serum AMH concentraction, protein and mRNA expression of AMH were measure with ELISA, immunohistochemical staining and qRT-PCR, respectively. Results The follicle and corpus luteum were atrophied after tripterygium wilfordii challenge, which was improved after treatment with Bushenyangxue prescription. Serum AMH, protein and mRNA expression of AMH were decreased tripterygium wilfordii-treated rats; this decrease was inhibited after treatment with Bushenyangxue prescription. Conclusions Our study indicates that Bushenyangxue prescription could preserve the AMH levels of POF rats. These findings suggest that Bushenyangxue prescription may be a useful strategy to treat POF.

    参考文献
    相似文献
    引证文献
引用本文

姬霞,傅金英,王冰玉,胡俊攀.补肾养血方对卵巢早衰大鼠中抗苗勒氏管激素的影响[J].中国比较医学杂志,2017,27(1):49~53.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2016-04-02
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2017-01-23
  • 出版日期:
自2024年1期开始,杂志参考文献改为中英文对照,具体格式要求可置下载中心查看!
关闭