芍药甘草颗粒对斑秃样小鼠毛发生长、行为学及下丘脑-垂体-肾上腺轴的影响
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1.北京中医药大学,北京 100029;2.首都医科大学附属北京中医医院,北京 100010;3.中日友好医院,北京 100029

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54

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R-33

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Effects of Shaoyao Gancao granule on hair growth, behavior, and the hypothalamic pituitary adrenal axis in mice with alopecia areata
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Affiliation:

1. Beijing University of Chinese Medicine, Beijing 100029, China.2. Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010.3. China-Japan Friendship Hospital, Beijing 100029

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    摘要:

    目的 探讨芍药甘草颗粒对斑秃样小鼠毛发生长、行为学及下丘脑-垂体-肾上腺轴的影响。 方法C3H/ HeJ 小鼠 42 只,随机分为空白对照组、模型组、芍药甘草颗粒高、中、低剂量组、促肾上腺皮质激素释放激素 1型受体拮抗剂组、复方甘草酸苷片组,每组 6 只,分别予以不同药物干预。 进行照片和毛发镜拍摄、体重称量、行为学测定,以及促肾上腺皮质激素释放激素、促肾上腺皮质激素、皮质醇、糖皮质激素受体、脑源性神经营养因子检测。 结果 与模型组比较,高剂量芍药甘草颗粒可加速斑秃样小鼠毛发再生及体重增长(P<0. 05),增加旷场实验运动总路程及中央区运动路程占总路程百分比(P<0. 05),减少强迫游泳实验及悬尾实验不动时间(P<0. 05),降低外周血促肾上腺皮质激素释放激素、促肾上腺皮质激素、皮质醇水平(P<0. 05),增加海马体糖皮质激素受体及脑源性神经营养因子表达(P<0. 05)。 结论 芍药甘草颗粒能促进斑秃样小鼠毛发生长、改善其行为学表现,其作用的发挥可能与下调促肾上腺皮质激素释放激素、促肾上腺皮质激素、皮质醇水平,上调糖皮质激素受体、脑源性神经营养因子表达,抑制下丘脑-垂体-肾上腺轴过度活化相关。

    Abstract:

    Objective To explore the effects of Shaoyao Gancao granule on hair growth, behavior, and the hypothalamic pituitary adrenal (HPA) axis in mice with alopecia areata (AA). Methods Forty-two C3H/ HeJ mice were randomly divided into control, model, Shaoyao Gancao granule high-, middle-, low-dose, corticotropin-releasing hormone receptor 1 ( CRHR1) antagonist, and compound glycyrrhizin tablet ( CGT) groups. Photography, dermoscopy, weight analysis, and behavioral measurement were performed. Corticotropin-releasing hormone ( CRH ), adrenocorticotropic hormone (ACTH), cortisol, glucocorticoid receptor ( GR), and brain-derived neurotrophic factor ( BDNF) were also assessed. Results Compared with the model group, high-dose Shaoyao Gancao granules improve hair regeneration and weight gain (P<0. 05), increased the percentage of total exercise distance and central area exercise distance in an open field test (P<0. 05), reduced the immobility time in the forced swimming and tail suspension experiments (P<0. 05),reduced peripheral blood levels of CRH, ACTH, and cortisol (P<0. 05), and increased expression of GR and BDNF in the hippocampus ( P<0. 05 ). Conclusions Shaoyao Gancao granule promotes hair growth and improves behavioral performance in mice with AA. These effects may be related to downregulating CRH, ACTH, and cortisol expression, upregulating GR and BDNF expression, and inhibiting excessive activation of the HPA axis.

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曲保全,吕书影,林文君,杨顶权.芍药甘草颗粒对斑秃样小鼠毛发生长、行为学及下丘脑-垂体-肾上腺轴的影响[J].中国比较医学杂志,2024,34(5):~46.

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  • 收稿日期:2023-08-25
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  • 在线发布日期: 2024-06-26
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