恒古骨伤愈合剂对db/ db 小鼠的降血糖和改善肠道菌群作用研究
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1.云南省执业药师注册中心,昆明 650101;2.昆明医科大学实验动物学部,昆明 650500;3.云南省第一人民医院急诊创伤外科,昆明 650031;4.昆明医科大学技术转移中心,昆明 650500

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R-33

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Effect of Osteoking on lowering blood sugar and improving intestinal flora in db / db mice
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1.Licensed Pharmacist Registration Center of Yunnan Province, Kunming 650101, China. 2.Laboratory Animal Science Department, Kunming Medial University, Kunming 650500. 3.Emergency Trauma Surgery, the First People’s Hospital of Yunnan Province, Kunming 650031. 4.Technology Transferring Center, Kunming Medical University, Kunming 650500

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    摘要:

    目的 探索恒古骨伤愈合剂对db/ db 小鼠血糖和肠道微生物的影响。 方法 将野生型小鼠作为对照组,db/ db 小鼠随机分为模型组和恒古骨伤愈合剂治疗组;灌胃给药12 周后,检测空腹血糖、血清糖化血红蛋白和胰岛素含量,使用16S rDNA 技术分析小鼠肠内容物菌群变化及预测菌群功能。 结果 与模型组比较,恒古骨伤愈合剂降低db/ db 小鼠空腹血糖(P<0. 01)、血清糖化血红蛋白(P<0. 01)、胰岛素抵抗指数(P<0. 01),升高胰岛素含量(P<0. 01);恒古骨伤愈合剂增加db/ db 小鼠盲结肠有益菌丰度,降低害菌丰度,其中马文氏菌属的丰度显著升高(P<0. 01);恒古骨伤愈合剂抑制了db/ db 小鼠菌群D-精氨酸和D-鸟氨酸代谢、鞘脂代谢、半乳糖代谢的降低及赖氨酸降解、硫中继系统、丙酸代谢的升高(P<0. 05)。 结论 恒古骨伤愈合剂具有降血糖功效和调节db/ db 小鼠肠道微生态平衡的作用。

    Abstract:

    Objective To investigate the effects of Osteoking on hyperglycemia and regulating gut microbiota in db/ db mice. Methods Wildtype mice were used as the control group, and db/ db mice were randomly divided into model and Osteoking groups. After intragastric administration for 12 weeks, fasting blood glucose and serum glycosylated hemoglobin and insulin levels were measured, changes in intestinal microflora were determined, and functional pathways related to intestinal microflora in mice were predicted by 16S rDNA sequencing. Results Compared with the model group, Osteoking decreased fasting blood glucose (P< 0. 01), serum glycosylated hemoglobin (P< 0. 01), and the insulin resistance index (P<0. 01), and increased insulin content (P<0. 01) in db/ db mice. Osteoking increased the abundance of beneficial intestinal microflora and decreased the abundance of harmful bacteria. Moreover, the abundance of Marvinbryantia was increased. Osteoking alleviated the decrease in metabolism of D-arginine and D-ornithine, sphingolipid, and galactose metabolism (P<0. 05) and inhibited lysine degradation, the sulfur relay system, and propanoate metabolism (P< 0. 05). Conclusions Osteoking has hypoglycemic properties and improves the intestinal microflora imbalance in db/ db mice.

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邓绍友,赵玉兰,王佩锦,李 蓉,赵宏斌,角建林,郑 红.恒古骨伤愈合剂对db/ db 小鼠的降血糖和改善肠道菌群作用研究[J].中国比较医学杂志,2023,33(12):28~33.

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  • 收稿日期:2023-02-10
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  • 在线发布日期: 2024-01-22
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