Objective To establish an effective and reliable chronic alcoholic brain injury model in mice. Methods Forty C57BL/6J mice were randomly assigned to a control group or a model group. Mice in the model group were given free access to 5% (v/ v) alcohol in drinking water and were intragastrically administered 28% (v/ v) alcohol. The gavage dosage increased gradually over the first two weeks (from 0 g/ kg to 6 g/ kg body weight) and remained at 6 g/ kg body weight for the subsequent four weeks. Mice in the control group were provided with normal water and given the same amount of saline via gavage. At the end of the experiment, the cognitive function and motor ability of the mice were evaluated through behavioral tests. Morphological changes in the brain tissue of mice were examined by histopathological staining. Results Compared to mice in the control group, mice in the model group showed cognitive impairments and motor dysfunction in the behavioral tests. Pathological examination of brain tissue from the model group mice showed morphological damage and cell necrosis in the hippocampus. Conclusions A mouse model of chronic alcoholic brain injury was effectively established in this study, providing a valuable tool for investigating the underlying mechanisms of and potential drug interventions for chronic alcoholic encephalopathy.