鼠伤寒沙门氏菌S.Typhimurium感染C57BL/6小鼠肠道模型的建立及表型分析
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国家自然科学基金(81601375),协和青年基金资助,中央高校基本科研业务费专项资金资助(3332016077),中国医学科学院医学实验动物研究所基本科研业务费专项资助(2016ZX310033)。


Etablishment and phenotypic analysis of a C57BL/6 mouse model of S. Typhimurium infection
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    摘要:

    目的 建立鼠伤寒沙门氏菌S.Typhimurium感染C57BL/6小鼠肠道模型。方法 先用5% NaHCO3溶液灌胃,中和部分胃酸,提高鼠伤寒沙门氏菌S.Typhimurium的感染能力,随后进行鼠伤寒沙门氏菌S.Typhimurium灌胃攻毒。观察小鼠的临床症状,对小鼠的生存情况及体重进行统计学分析,并通过组织病理切片分析小鼠肠道组织病理变化等。结果 鼠伤寒沙门氏菌S.Typhimurium攻毒后C57BL/6小鼠出现体重下降、萎靡不振、寒颤及死亡等表型,解剖学水平显示小鼠肠道充血膨胀。病理分析结果显示小鼠小肠绒毛间质水肿、肠粘膜层坏死及炎性细胞浸润等。结论 成功建立鼠伤寒沙门氏菌S.Typhimurium感染C57BL/6小鼠肠道模型,该模型在研究相关免疫分子或细胞因子在S.Typhimurium引发肠炎过程中的生物学功能及治疗等方面具有重要的科学意义。

    Abstract:

    Objective To establish a C57BL/6 mouse model of intestinal infection induced by S. Typhimurium. Methods In order to improve the infectious sensitivity of S. Typhimurium, C57BL/6 mice were intragastrically given 5% (w/v) NaHCO3. Then mice were challenged with S. Typhimurium. The health condition, survival and body weight of mice were observed from day 0 to day 7 after the bacterial infection. The pathological changes were also examined. Results the mice challenged with S. Typhimurium showed decreased body weight and typical clinical signs, including in appetence, piloerection and low survival rate. Macroscopic dissection revealed that intestinal hyperemia and swelling were founded in the mice challenged with S. Typhimurium. Histopathology showed intestinal epithelial and mucosal damages. Conclusions We have successfully established a C57BL/6 mouse model of S. Typhimurium infection. This model may be of crucial significance for studying the biological functions of associated immunological molecules or cytokines in the process of inflammatory bowel disease induced by S. Typhimurium.

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韦荣飞,李梦媛,徐大模,高苒.鼠伤寒沙门氏菌S. Typhimurium感染C57BL/6小鼠肠道模型的建立及表型分析[J].中国比较医学杂志,2017,27(6):33~36,45.

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  • 最后修改日期:2017-02-15
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  • 在线发布日期: 2017-07-08
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