Abstract:Neuralepithelial transplantation plays a critical role in axon pathfinding of motor neural and migration of neural crest cells during early development of vertebrate embryo. The animal model of chick embryo and chick-quail chimera was used to clarify a mature method of neuralepithelial transplantation in our study. Many drawbacks such as embryo death, less repeatability and cumbersome processes etc. exist in traditional transplantation. Hence, the advantages of foreign microsurgery transplantation were used as the mirror, as well as our trial-and error in our methods. Embryo pre-treatment, microneedle preparation and some details were present in this paper to highlight how to achieve a successful micro-transplantation. The protocol will dedicate to developmental neurobiology, such as migration of neural stem cells and axon pathfinding research early embryo development.