Objective By using loop-mediated isothermal amplification (LAMP) assay, to found the fast, convenient, sensitive and specific method of enterohaemorrhagic escherichia coli (ETEC), and evaluating the specificity and sensitivity of the method, for detection and diagnosis of bacterial diarrhea in laboratory animals. Methods According to the published LT toxin gene sequences (S60731.1) to design PCR and LAMP primers, the LAMP specificity and sensitivity compared with PCR. Results the LAMP method established detection limit was 100 pg, sensitivity is more 10 times than PCR and has the high specificity. Using PCR and LAMP to detect 27 feces samples of monkey diarrhea, we had the same results of LAMP (within 60 minutes) and PCR. And LAMP(within 90 min) has more sensitive than PCR. Conclusion we had established the ETEC LAMP detection method, this method is strong specificity, high sensitivity, fast and convenient, suitable for rapid detection of ETEC clinical.