Objective To study the effects of the mice UNCV protein on Hela cells apoptosis. Methods The recombinant plasmids containing Uncv gene of BALB/C mice were transfected into Hela cells. The Hela cells which stably overexpressed UNCV protein were selected. The effects of overexpressed UNCV protein on apoptosis of Hela cells induced by doxorubicin and serum starvation were measured by cell counting and flow cytometry. Results The Hela cells which stably overexpressed UNCV protein were obtained. The results of cell counting and flow cytometry showed that overexpression of the UNCV protein inhibited apoptosis of Hela cells induced by serum starvation. The effects of UNCV protein on apoptosis of Hela cells induced by doxorubicin were not significantly different. Conclusions Overexpression of UNCV protein inhibited apoptosis of Hela cells induced by serum starvation.