【Abstract】 objective To investigate the antidepressant like effect of DS-1226 on the chronic restraint rats and provide experimental data for the research of anti-depressant drugs. Methods 90 rats randomly divided into control group, model group, 3 treated group (18.75mg/kg,37.5mg/kg, 75mg/kg of DS-1226）, and 100mg/kg of citalopram group. 7 days after DS-1226 and citalopram were administrated orally, rats were restraint 14 hours per day for 28 days consecutively. Sucrose Preference, Open-field test, Novel object test and body weight measuring were used to evaluate the antidepressant like effect of DS-1226 on the chronic restraint rats. All the rats were sacrificed to measure the cortisone levels in serum at 32th day. Results The sucrose consumption（%）, explore behavior , spontaneous activity and the body weight of model rats were significantly reduced and cortisone levels in serum was increased . However, 37.5mg/kg ,75mg/kg DS-1226 and citalopram can increase sucrose consumption（%）, nose pokes(n), nose poke time(s), spontaneous activity especially the movement in centre area as well as body weight, and reduce latent time(s),cortisone levels in serum of chronic restraint rats. Conclusions DS-1226 showed the antidepressant like effect on the chronic restraint rats, which maybe used as antidepressant agents after further research. 【Key words】DS-1226 ; chronic restraint ; depression ; rats.