Effects of Apoptosis and DNA Damage Induced by Fluoride in rats Hepatocytes
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    Abstract:

    Objective To explore the liver cell apoptosis and DNA damage of rats exposed to fluoride. Method SD rats were randomly divided into control group, low fluorine group, middle fluorine group and high fluorine group,each group of 12. SD rats were incubated with 0, 50,100,and200 mg/ L sodiumfluoride for 120d. To observate the changes of dental, determinate the urinary fluoride in rats by using fluoride ion selective electrode method, observe the pathological changes by HE staining tissue ,test the DNA damage by comet assay,and measure liver cell apoptosis by Flow cytometry. Results Urinary fluoride is 23.52?.91、30.16?.78、61.23?.98 mg/L in each fluoride groups,and the fluoride groups were significantly higher than 0.07?.02 mg/L of the control group .The difference was statistically significant(p<0.01).Different doses of fluoride In rat liver cells showed varying degrees of swelling and pathological changes .The rat liver cell tailing rate and tail length in the fluoride group were significantly higher than those of the control group, and the difference was statistically significant . Liver cell tailing rate and tail length increase with fluoride dose increasing.The rates of cell apoptosis in the fluoride groups were significantly higher than that of the control group,and the rates of cell apoptosis in middle fluorine group and high fluorine group were significantly higher than that of low fluorine group(p<0.01).Conclusion Fluoride exposure induces DNA damage and apoptosis of the liver cell.There is a significant positive correlation between DNA damage and apoptosis induced by fluoride in certain level range.

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History
  • Received:November 28,2012
  • Revised:December 24,2012
  • Adopted:January 20,2013
  • Online: July 04,2013
  • Published: