Study of Estrogen Receptor α, Estrogen receptor β Immunohistochemical Localization in Beagles Ovary and Uterus
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The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)

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    Abstract:

    【Abstract】 Objective To examine the immunohistochemical expression of estrogen receptor α (ERα) and estrogen receptor β (ERβ) in the different cell types of ovary and uterus in Beagle Bitches. Methods Immunohistochemical SP method was used to detect the expression of ERα, ER β in dog uterus and ovary with DAB, BCIP/NBT and AEC staining. Results The Beagles ERα was mainly expressed in the nuclei of granulosa cells, ovarian interstitial gland epithelial cells and endometrial glandular epithelial cells (along with weaker staining in the cytosol). It was also expressed sporadically in the nuclei of the theca cells, the glands stromal cells, the arteriolar vascular endothelial cells, the artery smooth muscle cells and the small vein endothelial cells. On the contrary, ERβ was mainly expressed in the cytoplasm of all of the above cells (along with sporadic staining in the nucleus). In additionally, in the corpus luteum of different physiological periods, ERα was immunohistochemical positive in the nuclei of theca cells, and also in the nuclei and cytoplasm of luteinizing granulosa cells, while ERβ was still positive expressed in the cytoplasm of luteal cells. Coexpression of ERα and ERβ in dog uterus was not detected with BCIP/NBT and AEC double staining. Conclusion Immunohistochemical expression of ERα and ERβ in Beagles uterus and ovary was different, ERα mainly located in the nucleus of different cell types (along with weaker staining in the cytosol), whereas ERβ was mainly localized in the cytoplasm of the different cell types (along with sporadic staining in the nucleus).

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History
  • Received:November 19,2012
  • Revised:December 17,2012
  • Adopted:December 30,2012
  • Online: April 18,2013
  • Published: