The establishment of SH-SY5Y cell line stably expressing of beta-synuclein
DOI:
Author:
Affiliation:

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    【Abstract】 objective To establish the SH-SY5Y cell lines stably expressing of beta-synuclein protein. Methods SH-SY5Y cells were transfected by pcDNA3.1-β-synuclein plasmid using Lipofectin and selected with Zeocin. The expression of β-synuclein was detected by immunohistochemistry, situ immunofluorescence and Western blot. The effects of β-synuclein expression in SH-SY5Y cells on cell proliferation were detected by MTT assay. Results The stable expression of β-synuclein in SH -SY5Y cells was detected to be higher than the control group stably expressing β-Galactosidase by immunofluorescence, immunohistochemistry and Western blot. The cell proliferation was significantly increased compared with the control group by MTT assay(P<0.05). Conclusion β-synuclein was successfully expressed and exerted protective effect in SH-SY5Y cells ,which provide a foundation for the further study of β-synuclein in Parkinson's disease.

    Reference
    Related
    Cited by
Get Citation
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:October 29,2012
  • Revised:November 20,2012
  • Adopted:December 08,2012
  • Online: April 18,2013
  • Published: