Abstract:Objective: To establish the Non-alcoholic Fat Liver Animal Model of Microtus fortis and to observe the dynamic variation of relevant index. Methods: 70 6-week-old male Microtus fortis were randomly divided into two groups. The voles of model group were fed on the high-fat ingredients and the voles of control group were fed on the high-fiber diet. Voles of each group were sacrificed at 2, 4, 6, 8, 12 week. The body weight and liver weight were measured; serum values of ALT, AST, ALP, r-GT, CHE, TG, TC, FFA, GLU, HDL and LDL were calculated by biochemistry analyzer. The histological changes of livers were observed with light microscopy. Results: Voles fed the high-fat diet developed panlobular macrovesicular steatosis, whereas those fed the high-fiber diet had normal livers. Compared with the control group, liver weight and liver index of model group were significantly increased, serum ALT, AST, r-GT, CHE, TC, FFA, GLU and LDL were significantly high; HDL and TG were significantly low. Conclusions: A Non-alcoholic fatty liver model of Microtus fortis was successfully established by fed high-fat diet for 6 weeks, which could provide a useful tool to elucidate the pathogenesis and the treatment of the NAFL.