Effect of sheep bone collagen peptide on immune function in rat peritoneal macrophages
Author:
Affiliation:

College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Objective To investigate the effect of sheep bone collagen peptide ( SBCP) on the immune function of macrophages with or without stimulation with lipopolysaccharide (LPS). Methods Rat peritoneal macrophages were treated with different doses of SBCP, with or without stimulation with LPS. The metabolic activity and phagocytic ability of macrophages were measured by MTT assay and the neutral red method , respectively. The production of NO was measured with the Griess reaction, and TNF-α and IL-6 were detected by enzyme-linked immunosorbent assay (ELISA). The mRNA expression levels of key genes in the Toll-like receptor pathway and the negative regulatory factor Single Ig IL-1-related receptor were measured by qRT-PCR. Results SBCP treatment of normal macrophages increased their metabolic activity, phagocytosis, NO, TNF-α and IL-6 production, and upregulated mRNA expression of key genes in the TLR pathway and downregulated SIGIRR mRNA expression in a dose-dependent manner. At a concentration of 105 μg / mL, there was no significant difference between the SBCP and LPS group. SBCP treatment of LPS-stimulated macrophages inhibited their metabolic activity, with 102 μg / mL having the greatest effect, while 103 μg / mL most strongly inhibited phagocytosis and the secretion of NO, TNF-α and IL-6. This concentration also downregulated the mRNA expression of TLR-related genes and upregulated the mRNA expression of SIGIRR. Conclusions SBCP has a dual regulatory effect on the inflammatory response through the TLR signaling pathway. At a concentration of 10 ~ 104 μg / mL, it enhances the immune activity of macrophages, while at 105 μg / mL, it induces macrophages to release various inflammatory mediators. However, SBCP inhibits the inflammatory reaction induced by LPS.

    Reference
    Related
    Cited by
Get Citation
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:October 28,2020
  • Revised:
  • Adopted:
  • Online: June 02,2021
  • Published: