Tumor necrosis factor (TNF) is essential for the development and control of tuberculosis. The zebrafish- marine mycobacterium model is one of a growing number of models currently used in tuberculosis research. This model can help us to explore the relationship between TNF and macrophages, granuloma, or mycobacterium, so as to better understand the relevant factors affecting TNF production and the role of TNF in the course of tuberculosis.