Application of reduced glutathione in biological purification and rapid propagation of AG129 mice
Received:July 17, 2019  
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DOI:10. 3969 / j.issn.1005-4847. 2020. 01. 014
KeyWord:AG129 mice; glutathione; biological purification; rapid propagation
张帆 中国科学院武汉病毒研究所, 武汉
安学芳 中国科学院武汉病毒研究所, 武汉
赵赫 中国科学院武汉病毒研究所, 武汉
李丽 中国科学院武汉病毒研究所, 武汉
肖宇宙 中国科学院武汉病毒研究所, 武汉
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      Objective AG129 mice have difficulty giving birth naturally, and conventional in vitro fertilization (IVF) reagents do not yield a sufficient number of embryos for transplantation. Reduced glutathione (GSH) was used to regulate a component of the IVF reagent and complete the IVF process in AG129 mice, presenting a potential method for biological purification and rapid reproduction of genetically engineered mouse strains with IVF difficulties. Methods Four experimental groups were designed using 0 mmol / L (control group), 0. 5 mmol / L, 1 mmol / L and 2 mmol / L of GSH for sperm capacitation and fertilization. Each group contained 5 superovulated mice, and the assay was repeated three times. Results The average fertilization rate differed between the GSH experimental groups and the control group (P< 0. 05). The 1 mmol / L GSH concentration had the best IVF effect on the AG129 mice. The average fertilization rate was (43. 35 ± 1. 76)%, and the embryo status was good. All recipient mice became pregnant and gave birth successfully after the embryos were transferred. Conclusions A GSH concentration of 1 mmol / L significantly improves the IVF efficiency of AG129 mice. This provides a potential solution for the biological purification and rapid propagation of mice with IVF difficulties.
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