Dairy products supplement calcium and promote intestinal motility in zebrafish models
Received:December 04, 2019  
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DOI:10. 3969 / j.issn.1005-4847. 2020. 01. 004
KeyWord:zebrafish; dairy products; calcium supplementation; intestinal peristalsis
蒋一之 南京新环检测科技有限公司,南京
朱晓宇 杭州环特生物科技股份有限公司, 杭州
李露露 1.南京新环检测科技有限公司,南京 ; 2. 杭州环特生物科技股份有限公司, 杭州
俞航萍 杭州环特生物科技股份有限公司, 杭州
荣佳 南京新环检测科技有限公司,南京
李春启 1.南京新环检测科技有限公司,南京 ; 2. 杭州环特生物科技股份有限公司, 杭州 ; 3. 浙江省人用物品安全性评价技术研究重点实验室早期评价基地, 杭州
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      Objective To validate and compare the functions of calcium supplementation and intestinal peristalsis promotion between two brands of dairy products using zebrafish models. Methods Zebrafish embryos at 3 days post fertilization (dpf) were selected and exposed to UHT(ultra heat treated) milk at 0 (blank control), 2. 5, 5. 0, and 10. 0 mg / mL for 96 h. After treatment, zebrafish were stained with calcein and observed under a stereofluorescent microscope to measure the fluorescence intensity of vertebrae. Zebrafish were also fed with Nile red at 5 dpf for 16 h, followed by a 24 h exposure to the same concentrations of yogurt and lactobacillus beverage. Intestinal tract fluorescence was measured to assess the ability of intestinal peristalsis promotion. Results UHT milk, yogurt, and lactobacillus beverage all increased blank control, 2 of the 6 types of UHT milk increased vertebrate fluorescent intensity; and 8 types of yogurt and lactobacillus beverage significantly elevated intestinal motility, although yogurt was better than the lactobacillus beverage. Conclusions The maximum tolerable concentration (MTC) of UHT milk, yogurt, and lactobacillus beverage is 10. 0 mg / mL in zebrafish. These dairy products have the same functions in zebrafish as in humans in term of calcium supplementation and intestinal peristalsis effect.
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