Objective To observe the various parameters related to metabolic syndrome and prediabetes in aLeptin receptor-knockout ( Lepr ^{-/ -} ) rat model established by CRISPR/ Cas9 technique. Methods Periodically, wemeasured the weights, daily food intake, random and fasting blood glucose, and serum biochemical indexes of the Lepr ^{-/ -}rats, and performed glucose and insulin tolerance tests. Pathological changes in the main organs of Lepr ^{-/ -} rats wereexamined at 14 and 18 weeks of age. We also observed the lipid deposition in the main organs of Lepr ^{-/ -} rats. Results Lepr ^{-/ -} rats exhibited obesity, high food intake, dysfunctional glucose tolerance and insulin resistance as well asdyslipidemia. Islet and myocardial hypertrophies, fatty liver, and obesity-related glomerulopathy were observed in the Lepr ^{-/ -} rats at 14 and 18 weeks of age. Lipid deposition was found in hepatocytes, renal tubular epithelial cells, and skeletalmuscle cells. Conclusions Lepr ^{-/ -} rats are an ideal animal model of metabolic diseases and may contribute to revealing the pathogenesis and new drug development for such metabolic disorders.