Effects of high-fat diet feeding time on the establishment of a rat model of type 2 diabetic nephropathy
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(Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700, China)

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Beijing Natural Science Foundation(No. 7162123)

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    Abstract:

    Objective To investigate the effects of different high?fat diet feeding time durations on blood glucose (BG), insulin resistance index (HOMA-IR), and urinary albumin excretion rate (UAER) in rats with high fat diet induced type 2 diabetic nephropathy (DN). Methods Unilateral renal artery ligation, high-fat diet (throughout the experiment period), and low dose streptozotocin (STZ) intraperitoneal injection were used to establish a type 2 DN rat model. After the operation, rats in the DN1 and DN2 groups received an intraperitoneal injection of STZ 30 mg/ kg after 4 and 8 weeks of high-fat diet feeding, respectively. UAERs of the DN1 and DN2 groups were compared at 4 weeks after the STZ injection and the end of study (EOS). BG, body weight, HOMA-IR, kidney index, and pathological changes of the kidney were observed. Results UAER was increased in both groups at 4 weeks after the STZ injection, but significantly higher in the group DN2 than in the DN1 group ( P <0.01). At the end of study (the 12th week), the renal tissues showed pathological changes, including glomerular capillary loop hypertrophy, increased mesangial matrix, and decreased capsule space in both groups. Compared with the DN1 group, the body weight was significantly higher ( P <0.01), kidney index was significantly lower ( P <0.01), while BG, serum insulin level, HOMA-IR, and UAER were no significantly changed in the DM2 group ( P >0.05, respectively). Conclusions The results show that extending the feeding time of high-fat diet can aggravate the kidney damages in diabetic rats, but it can also delay the start of any planned intervention. Therefore, the experimental protocol should be carefully designed based on the study objective .

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History
  • Received:July 14,2017
  • Revised:
  • Adopted:
  • Online: March 06,2018
  • Published: