Objective To establish a mouse model of aorta dissection (AD) by β-aminopropionitrile (BAPN) in drinking water + subcutaneously pumped angiotensin Ⅱ (Ang Ⅱ) infusion. Methods Forty 3-week-old C57B1/6J male mice were randomly divided into two groups. All animals received 0.1 g/kg/d BAPN in drinking water for 4 weeks. Then the BAPN drinking + saline infusion group and BAPN drinking + Ang Ⅱ infusion group received continuous saline or Ang Ⅱ (1,000 ng/kg/min) infusion, respectively, via subcutaneous osmotic minipump for 72 hour. The mice were restricted in a noninvasive computerized tail-cuff system and their arterial systolic blood pressure and heart rate were monitored. Autopsy was performed if a mouse died during the experiment. At the end of the experiment, mice were sacrificed by injection with an overdose of sodium pentobarbital and the aortas were harvested. The formation of aortic false lumen was observed by pathology using hematoxylin-eosin staining. Results The overall incidence of AD in the BAPN drinking administration +Ang Ⅱ infusion group was 95%, whereas the incidence of AD in the BAPN drinking administration +saline infusion group was only 5%. The mortality from dissecting aneurysm rupture was 24% in the BAPN drinking administration +Ang Ⅱ infusion group during the experiment. Pathological examination of the aortic cross-sections clearly showed the formation of blood-filled false lumens induced by Ang Ⅱ. Conclusions A mouse model with high incidence of aortic dissection is successfully established.