Abstract:Objective To establish animal models of functional dyspepsia with spleen deficiency and to compare the efficacy of different methods. Methods Rat models were established by iodoacetamide(IA)-treatment or combined with swimming.Appearance,body weight,food intake of the rats were observed,and serum motilin,cholecystokinin,lactate,gastrin content and urinary D-xylose excretion rates were detected to confirm whether the model of functional dyspepsia with spleen deficiency was established. Results The IA-treated rats had less food intake and a slower body weight gain.The IA-treated combined with swimming rats presented spleen-hypofunction symptoms,such as emaciation,hair dry and loose stools, their urinary D-xylose excretion rate, serum motilin, gastrin content were decreased, and serum cholecystokinin and lactate contents were increased significantly (P<0.05 for all). Conclusions All the three methods used in this study can result in symptoms of functional dyspepsia with spleen deficiency.However,IA-treatment combined with swimming models appear more close to spleen deficiency-like presentation, and the best model is the IA-treated combined with platform standing.