Effect of expression of PP1γ and DNA methylation on learning and memory in mice
Author:
Affiliation:

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Objectives To study the role of protein serine/threonine phosphatase1γ (PP1γ) and DNA methylation in learning and memory. Methods The mice and cells were treated with 5-aza-cdR, a DNA methyltransferase (DNMT) inhibitor, and to observe the changes of ability of learning and memory and expression levels of PP1γ in mice. The ability of learning and memory in mice was assessed by Morris Water maze test. The mRNA expression levels of DNMTs and PP1γ in the mouse hippocampus were determined by real-time PCR and the protein level of PP1γ was measured by Western blot. To further investigate their role, NG108-15 cell line was treated with 5-aza-cdR. Flow cytometry, xCelligence and luciferase were used to detect the effects of 5-aza-cdR on the proliferation, apoptosis and PP1γ transcription in the NG108-15 cells. Results The ability of learning and memory was enhanced in the mice after administration of 5-aza-cdR injection. The expressions of DNMTs and PP1γ were decreased in the mouse hippocampus after injection of 5-aza-cdR. On the other side, 10 μmol/L 5-aza-cdR inhibited cell proliferation and decreased PP1γ transcription without inducing apoptosis. Conclusions Our data demonstrate that 5-aza-cdR inhibits the expression of PP1γ which is related to learning and memory in mice.

    Reference
    Related
    Cited by
Get Citation
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:August 30,2016
  • Revised:
  • Adopted:
  • Online: April 28,2017
  • Published: