Objective To investigate the effects of P-selectin gene knockout on the biological characteristics of mice. Methods P-selectin knockout (PKO) mice and C57BL/6 mice were housed in SPF environment. To make sure that the mice were pure PKO mice by gene identification. To determine the general condition, the activity status, reproduction of P-selectin knockout mice were observed and tested. HE staining was used to observed the histological morphology and structures of 6-week-old and 18-week-old P-selectin knockoutout mice. Results PKO mice and C57 mice have similar multiplication, there was no significant difference in reproduction and blood routine test. The stuctures of liver, spleen, lung and kidney were normal. Conclusions P-selectin knockout has no significant effects on reproduction, blood routine test and major organs.This research provides animal model theoretical basis for further study on the effects of P-selection in diseases.