Objective In order to lay the foundation for establishment of a clean grade Meriones meridianus population, and provide reference for the formulation of Meriones meridianus parasite detection standard. Methods A survey of parasite infection in Meriones meridianus was carried out, and three kinds of antiparasitic drugs, tetramethrin/permethrin, praziquantel, and tinidazole were used in high-, medium- and low-doses for eradication of the target parasites. Results Three types of parasites were detected among the animals. The infection rate of surface parasite Myobia musculi was 82.1% (23/28), that of intestinal parasite Hymenolepis nana was 78.1% (25/32) and Tritrichomonas muris was 90.6% (29/32). Significant results of parasite eradication were achieved with the antiparasitic drugs. Medicated bath using 1% tetramethrin/permethrin for 30 s every 4 days for 5 times and 1.5% tetramethrin/permethrin for 30 s every 4 days for 3 times completely eradicated the surface Myobia musculi infection. Praziquantel in a dose of 31 mg/kg by daily gastric gavage for consecutive 7 days was the optimal dosage to completely eradicate the intestinal Hymenolepis nana. Tinidazole in a dose of 42 mg/kg administered by gastric gavage daily for consecutive 7 days was the optimal dosage for eradication of intestinal Tritrichomonas muris infection. Conclusions Three types of parasites are detected in Meriones meridianus in our laboratory, i.e. surface infection with Myobia musculi, and intestinal infection with Tritrichomonas muris and Hymenolepis nana. Tetramethrin/permethrin, praziquantel and tinidazole can be used to effectively eradicate these three types of parasites, respectively.