Effect of paeoniflorin on the calcium concentration in rat dorsal root ganglion neurons
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    Abstract:

    Objective To establish a method to evaluate the effect of paeoniflorin (PF)on free calcium (Ca2+)concentration in dorsal root ganglion (DRG)neurons in rats. Methods DRGs were obtained by microdissection, then were digested by trypsin and sieved.The dissociated neurons were alternately cultured and purified with DF-12 and anti-mitotic culture medium to obtain primary rat DRG neurons, and the purity of DRG neurons was measured by immunofluorescence technique. The fluorescence intensity of Ca2+ in DRGs was observed by laser confocal microscopy (LSCM)imaging, and the changes of fluorescence intensity were analyzed to explore the effects of PF on DRG intracellular free calcium concentration and capsaicin receptor. Results The purity of DRG cells isolated by the above mentioned method was up to 95%.Capsazepine(CAP)inhibited the increase of intracellular Ca2+ by blocking the capsaicin-activated transient receptor potential vanilloid 1 (TRPV1). PF exhibited the influx of extracellular Ca2+. Conclusions We would hypothesize that PF probably inhibits the significant increase of Ca2+ in DRG cells by acting on TRPV1 channel, and the method established in this study can be used to evaluate the effects of drugs on Ca2+ concentration in rat dorsal root ganflion neurons.

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History
  • Received:August 04,2015
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  • Online: March 05,2016
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