Objective To establish a loop-mediated isothermal amplification (LAMP) method for detecting diarrhea pathogens (Shigella and Salmonella) in rhesus monkeys and evaluate the application of the LAMP method for detecting bacterial diseases in non-human primate laboratory animals. Materials and Methods A total of 205 fecal samples of rhesus monkeys were detected in this LAMP assay. The specificity and sensitivity of LAMP for Shigella and Salmonella were analyzed, and real-time polymerase chain reaction (REAL-TIME PCR) assay was employed as control. Results The LAMP method established here needed only 45 min to complete the reaction at 63℃. Its detection limit was 10 pg/μL and with a high specificity. The positive rate of Shigella and Salmonella was 1.5% and 6.3%, respectively. Conclusions Here we have established a fast and simple Shigella and Salmonella LAMP detection method that has strong specificity and high sensitivity and is suitable for rapid detection of bacterial disease in macaques. The development of this rapid detection kit is underway, and it will be helpful to the diarrhea detection.