Objective To observe the influence of a Chinese traditional medicine, Shexiangbaoxinwan, on myocardial injury markers and C-reactive protein in exhaustively exercised rats. Methods A total of 52 male 11-12 week old Wistar rats were divided randomly into two groups: the experimental (n=26) and control groups (n=26).The two groups of rats took food and water freely. The experimental group of rats was given Shexiangbaoxinwan (2 capsules per day) for two weeks. Following a single bout of exhaustive swimming (≥2 hrs), the serum and myocardial CK, CK-MB, TnT, BNP and CRP in both groups were determined and compared. Results Compared with the control group, the exhaustive swimming time of the experimental group was significantly prolonged (P<0.01). The levels of serum and myocardial CK, CK-MB, TnT, BNP and CRP in the experimental group were significantly lower than those in the control group (P<0.05 for all). Conclusions The Chinese traditional medicine Shexiangbaoxinwan can significantly decrease the levels of rat serum and myocardial CK, CK-MB, TnT, BNP and CRP after a single bout of exhaustive swimming, and car alleviate myocardial injury.