Expression of mitochondrial apoptotic pathway-related factors in Sprague-Dawley rats with spontaneous mammary neoplasms
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    Abstract:

    Objective To investigate the expression of AIF, CYT C, PAF-1, caspase-3, and XIAP in Sprague-Dawley rats with spontaneous mammary neoplasms. Methods One-hundred and thirty 3-4-week old SPF Spargue-Dawley rats (♀:♂=1:1) were fed in a specific pathogen free (SPF) breeding barrier for 60 weeks. The occurrence of spontaneous breast tumors was recorded and histopathology was performed to identify the types of tumors. The rats were divided into 3 groups: rats with normal breast tissue (group I), with benign tumors (group II) and with malignant tumors (group III). The expression of AIF, CYT C, APAF-1, caspase-3 and XIAP proteins and mRNAs were detected by immunhistochemistry (IHC) and RT-PCR assay. Results Among these 130 SD rats, 14 rats were observed having spontaneous mammary neoplasms with the incidence rate of 10.77% (14/130). In these neoplasm cases, 7 cases were mammary fibroadenomas, 7 cases of breast carcinoma, both with an incidence rate of 5.38%. Immunohistochemistry showed that, compared with the group I, the positive expressions of AIF, APAF-1, caspase-3 were decreased significantly (P<0.01), and the CYT C and XIAP expressions were significantly increased in the group II. The positive expression of all genes except XIAP was decreased in the group III(P<0.01). Compared with the group II, APAF-1 and XIAP were significantly higher in the group III (P<0.01), and the positive expression of AIF, Cyt C, and caspases-3 were significantly decreased (P<0.01). In the results of RT-PCR assay, except APAF-1 which showed significant correlation with the results of immunohistochemistry (P<0.05), all the others showed an extremely significant correlation with immunohistochemical results (P<0.01). Conclusions Mammary tumors are most common spontaneous neoplasms in SD rats. Abnormal expression of mitochondrial apoptotic pathway-related factors AIF, CytC, APAF-1, caspase-3, and XIAP are correlated with the carcinogenesis and development of breast tumors.

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History
  • Received:January 27,2015
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  • Online: September 06,2015
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