Laboratory animal experiments of pathogenic infection play more and more roles in animal medicine and biological product research. We surveyed the scientific papers involved with poultry infection experiments published in NCBI Pubmed from Jan.1^st, 2009 through April 29^th,2014, focused on the "humane endpoint" and euthanasia. Among the 247 reports, we found that the euthanasia methods adopted by the researchers were diverse, though almost all authors claimed that they complied with the Animal Experiment Care and Welfare Committees. Carbon dioxide inhalation and anesthetic injection are the most commonly used ways, while the reagents and doses are various. Moreover, the judging standards for experimental or humane end-point vary across the experiments, there are still no standard routes or doses when using anesthetics yet. The paper also compares the characteristics of reported euthanasia methods.