Effect of circadian rhythm on local cerebral ischemia-reperfusion injury in rats
Author:
Affiliation:

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Objective To observe the effect of circadian rhythm on local cerebral ischemia-reperfusion injury in rats. Methods One hundred SPF male SD rats (body weight 320±20 g) were raised in light(6:00-18:00)-dark(18:00-6:00)cycle environment for 4 weeks. Then all rats were randomly divided into 6:00 group, 12:00 group, 18:00 group and 24:00 group. Rat models of cerebral ischemia-reperfusion injury were induced by middle cerebral artery occlusion at corresponding time points for each group. The neurological symptoms were evaluated, cerebral infarction area and SOD and MDA levels in brain tissue were determined, intracellar Ca2+ concentration of brain tissue was measured, the level of serum endothelin (ET) and VEGF were assayed, and the pathological changes of brain tissue at 24 h after ischemia-reperfusion were examined. Results All rats showed typical neurological symptoms, which were milder in the 24:00 group than that of the 6:00 group, and the cerebral infarction areas of the 6:00 group and 12:00 group were significantly larger than that of the 18:00 group and 24:00 group. The SOD activities of 18:00 group and 24:00 group were significantly higher than that of the 6:00 group and 12:00 group, and the MDA levels of 18:00 group and 24:00 group were significantly lower than that of the 6:00 group and 12:00 group. The concentration of intracellular Ca2+ of the 24:00 group was lower than that of 6:00 group and 12:00 group, and the concentration of intracellular Ca2+ of the 18:00 group was significantly lower than that of the 6:00 group. The contents of serum ET in the 18:00 group and 24:00 group were significantly lower than that in the 6:00 group. The content of serum VEGF of 24:00 group was obviously lower than that of the 6:00 group and 12:00 group. The brain pathological changes of the 18:00 group and 24:00 group were obviously milder than that of the 6:00 group and 12:00 group. Conclusion Circadian rhythm may significantly influence the cerebral ischemia-reperfusion injury in rats.

    Reference
    Related
    Cited by
Get Citation
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:September 06,2014
  • Revised:
  • Adopted:
  • Online: March 04,2015
  • Published: