Objective To observe the effect of high sucrose/high fat diet and streptozotocin (STZ) on glucose and lipid metabolism, liver histology and protein kinase B (PKB) phasphorylation in Chinese Banna miniature pigs, and to investigate its molecular mechanism.Methods Eight healthy 2-month old male Banna minipigs were randomly divided into control and diabetes groups, 4 pigs in each group. Diabetic minipigs were induced by high sucrose/high fat diet and STZ and the levels of fasting blood glucose, insulin, total cholesterol, triglycerides were measured per month for 12 months. At the end of 12^th month, the minipigs were sacrificed and liver histology was examined using HE staining and the hepatic lipid accumulation was observed using Sudan IV staining. Real-time PCR and Western blot were used to determine the expression of PKB mRNA and protein, and the phosphorylation level of PKB-Ser⁴⁷³ in the liver tissue. Results At the end of 12th month, the minipigs of model group showed hyperglycemia, insulin deficiency and dyslipidemia compared with that of the control group (P<0.05).The fat degeneration was increased, and glycogen was decreased in the liver tissue of model group. High sucrose/high fat diet and STZ increased the PKB mRNA and protein expression, while decreased PKB phosphorylation in the liver tissue (P<0.05). Conclusions High sucrose/high fat diet and STZ can induce insulin resistance (IR) and diabetes mellitus, promote lipid and cholesterol accumulation, and inhibite hepatic glycogenogenesis, which may be related with inhibition of PKB phosphorylation.