Analysis of the cloned IGF-1 gene cDNA sequence of Tibet minpig
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    Abstract:

    Objective To compare and analyze the sequence between Tibet minipigs and Pubmed pig (NM_214256.1) IGF-1 cDNA by cloning and sequencing the IGF-1 gene cDNA of the liver tissue of Tibet minipigs. Methods Total RNA was extracted from Tibet minipig liver and amplified the sequence of IGF-1 gene by RT-PCR. The amplified fragment was cloned into pMD18-T vector, and the recombinant plasmid pMD18-T-IGF-1 was constructed and sequenced. Results Tibet minipig IGF-1 gene fragment containing the coding region of 567 bp. The coding region encoded 186 amino acids, and 99% were homologous compared with that of the pig (NM_214256.1). Compared with pig (NM_214256.1), we found that the mutation in the 440 bp, 455 pb, occurred at G→A, C→T mutation, and the corresponding amino acids were changed, histidine→arginine and leucine→serine, respectively. Conclusions The results of this study provide a molecular basis for breeding research. Whether the mutation at the two sites changes the protein function and body size or not need to be further studied.

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History
  • Received:August 11,2014
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  • Online: March 04,2015
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