Objective To study whether the green fluorescent protein (GFP) gene can be successfully expressed in green fluorescent nude mice and the tissue distribution characteristics. Methods Small animal imaging system and RT-PCR assay were used to detect the GFP tissue distribution and fluorescence expression level. Results The GFP can be expressed in multiple tissues in green fluorescent nude mice. A higher expression was observed in the pancreas, heart, brain, and skin. Conclusion Exogenous GFP can be stably expressed and inherited in green fluorescent nude mice, with the highest expression in the pancreas.