Objective To establish normal kidney data by measuring the diameter and volume of the kidneys in adult Guizhou miniature pig. Methods Twelve healthy adult Guizhou miniature pigs (male 6, female 6) were used in this study. The kidneys were scanned by using Siemens dual source CT, and the raw data were used for multiplanar reconstruction (MPR) and volume reconstruction (VR) using the software Aquarius iNtuition 4.4. The anteroposterior and left to right dimensions of both kidneys were measured at the median transverse plane of the organ, and the long and short diameters of both kidneys were measured at the maximum coronal section of the organ. The volume of both kidneys and miniature pig itself, the ratio of long to short diameters of both kidneys, and the relationship between the ratio of long to short diameter and the kidney were calculated. The correlation between the length dimension, length diameter ratio and the volume of the kidney was analyzed, and the correlation between volume of the kidney and miniature pig was analyzed as well. P<0.05 was set as statistically significant. Results The dimensions of anteroposterior, left to right, long and short diameters of left kidney were 40.40±1.67 mm, 27.47±1.40 mm, 80.36±2.74 mm and 31.79±1.99 mm, respectively. The volume of left kidney was 52.78±2.25 cm³, and the ratio of long to short diameter of the left kidney was 2.42 to 2.71:1. The dimension of anteroposterior, left to right, long and short diameters of the right kidney were 40.95±1.43 mm, 26.90±0.65 mm,79.03±2.38 mm and 32.04±2.34 mm, respectively. The volume of right kidney was 51.91±2.25 cm³, and the ratio of long to short diameter of the right kidney was 2.33 to 2.77: 1. There were highly positive and significant correlations between the long diameter and the kidney volume (r_left=0.92, P<0.05; r_right=0.88, P<0.05) as well as between the kidney volume and the miniature pig (r_left=0.96, P<0.05; r_right=0.98, P<0.05), while the ratio of long to short diameter of the kidney was negatively related to the volume of the miniature pig (r_left=-0.51, P<0.05; r_right=-0.67, P<0.05). Conclusions Multislice spiral CT and its post-processing software can accurately measure various data of the kidneys of Guizhou miniature pig in vivo, providing normal reference of the animal model for the research of human kidney diseases.