Electrophysiological characteristics of the right and left atrial appendages in 48-h atrial fibrillation canine detected by microelectrode arrays chip
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    Abstract:

    Objective To explore the electrophysiological characteristics of right atrial appendage (RAA) and left atrial appendage (LAA) in 48-h atrial fibrillation (AF) canine detected by microelectrode array (MEA) chip technique. Methods Twelve adult healthy mongrel canine were randomly divided into two groups:48-h pacing group and control group. The 48-h AF model was established by rapid pacing the right atrium. The RAA and LAA were quickly removed and immersed into Tyrode’s solution in MEA chip dish. The morphology, duration, voltage, discharge frequency and conduction of field action potential were recorded. ResultsIn the AF group, the rhythms of LAA and RAA tissue field potential were irregular. The frequency of LAA (185.22±25.62 beats/min) was increased by 15.67% (156.44±8.88 beats/min)(P<0.01), and the frequency of RAA (102.39±16 beats/min) was decreased by 34.62% than that in the control group (156.44±8.88 beats/min) (P<0.01). The voltages of LAA (458.33±26.73)μV and RAA (504.83±39.93)μV were decreased than those of the control group (740.55±18.93)μV and (840.56±18.93)μV, respectively, (P<0.01). The durations of field potential of LAA (45.28±8.59)ms and RAA (61.78±7.1)ms were significantly shortened than those of the control group (70.77±6.98)ms (P<0.01). The electric impulse presented anisotropic alteration. ConclusionsMEA is a sensitive, long-lasting and stable technique, and local tissue action potential can be used to assess the electrophysiological characteristics of field potential in animal heart slices using multiple-channel recording mapping system. The discharge frequency of left atrial appendage is increased and frequency irregularity occurs after 48 h atrial fibrillation, the voltage of field potential is decreased in the atrial appendage, and the field potential duration is prolonged.

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