PiggyBac转座酶转基因小鼠模型的建立

马元武; 王冬梅; 廉虹; 王贵利; 张连峰

Establishment of a PiggyBac transposase-expressing transgenic mouse model

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DOI：10.3969/j.issn.1005-4847.2012.03.013

KeyWord:DNA transposon；PiggyBac；transposase；Transgenic mice

Author	Institution
马元武	中国医学科学院北京协和医学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,北京
王冬梅	中国医学科学院北京协和医学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,北京
廉虹	中国医学科学院北京协和医学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,北京
王贵利	中国医学科学院北京协和医学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,北京
张连峰	中国医学科学院北京协和医学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,北京

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Abstract:

Objective To establish a PiggyBac transposase-expressing transgenic mouse model for study of the genetic modification mediated by transposon in mice. MethodsPiggyBac transposase gene was driven by CMV promoter and transgenic mice were created by the microinjection. The gene type of transgenic line was identified by PCR and the gene expression level in testis was determined by RT-PCR. The activity of the PiggyBac transposase was detected with the transposition efficiency by Southern blot in the mice crossed with transposon donor transgenic mice. ResultsSeven lines of transposase transgenic mice were obtained by microinjection. One mouse line with relatively higher expression level of transposase in the testis was obtained and the transposase induced the transposition of the transposon donor DNA fragment in the mouse genome. ConclusionA PiggyBac transposase-expressing transgenic mouse model is successfully established. This model will greatly contribute to the research of the genetic modification mediated by transposon in mice. 